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 ORIGINAL ARTICLE
Year : 2007  |  Volume : 17  |  Issue : 1  |  Page : 7-9

Urine protein thiols in chronic renal failure patients


Department of Biochemistry, Kasturba Medical College, Manipal, India

Correspondence Address:
M Prakash
Department of Biochemistry, Kasturba Medical College, Manipal - 576 104
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0971-4065.35013

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Proteinuria is the most common finding in chronic renal failure (CRF). The thiol groups on proteins contribute most to the antioxidant status of the body. Serum protein thiols were found to be decreased in CRF. The current study was designed to find out the levels of such protein-bound thiols in the urine of patients with CRF. The study was conducted on urine and serum samples of 20 healthy controls and 40 CRF cases. Serum and urine protein thiol levels were determined by spectrophotometric method using dithionitrobenzoic acid (DTNB). Serum albumin, urine protein, and urine creatinine levels were estimated in 24-h urine samples by an automated analyzer. There was significant decrease in serum albumin ( P <0.01) and serum protein thiols ( P <0.01) in CRF patients compared to healthy controls. In the urine samples, there was significant increase in protein ( P <0.01) and decrease in protein-bound thiols ( P <0.01) in CRF cases compared to healthy controls. Serum albumin correlated positively with serum protein thiols (r = 0.561, P <0.01), and urinary protein correlated negatively with urinary protein-bound thiols (r = -0.410, P <0.009). Protein-bound thiols, the major antioxidants in the body, are decreased in CRF cases. Due to increased consumption of protein-bound thiols in such an oxidative environment, there was significant decrease in protein-bound thiols in urine.






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Indian Journal of Nephrology
Published by Wolters Kluwer - Medknow
Online since 20th Sept '07