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Letters to Editor
24 (
1
); 68-68
doi:
10.4103/0971-4065.125141

Utility of left-over renal tissue for light microscopy after immunofluorescence

Department of Pathology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India
Department of Surgical Oncology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India
Department of Nephrology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India
Address for correspondence: Dr. Rashmi Patnayak, Department of Pathology, Sri Venkateswara Institute of Medical Sciences, Tirupati - 517 507, Andhra Pradesh, India. E-mail: rashmipatnayak2002@yahoo.co.in
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This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Disclaimer:
This article was originally published by Medknow Publications & Media Pvt Ltd and was migrated to Scientific Scholar after the change of Publisher.

Sir,

We read the article “retrieval of kidney tissue for light microscopy (LM) from frozen tissue processed for immunofluorescence (IF): A simple procedure to avoid repeat kidney biopsies” by Tyagi et al., with the interest.[1] In our institute, we also usually receive two cores of renal tissue, one in buffered formalin for LM and the other in normal saline for IF. Usually, we process both the tissue simultaneously. We have received adequate renal tissue, most of the time. But occasionally, there are cases where we have faced a similar situation as described by the authors, where glomeruli were present in IF sections, but absent in LM sections. In those instances, we have processed the left over frozen tissue originally sent for IF by fixing it with formalin for 2 hours. In our experience, we also have observed that crescents are easily diagnosed in the processed left over frozen IF tissue, thereby helping in arriving at the diagnosis and avoiding the necessity of repeat renal biopsy. So, in our opinion, in cases where renal biopsy is inadequate in LM sections, the left over frozen tissue can be processed to look for glomeruli, before asking for repeat renal biopsy.

Acknowledgment

The authors wish to thank senior technicians Mrs. Ushanandini and Mr. Ramana for their help.

Reference

  1. , , , , . Retrieval of kidney tissue for light microscopy from frozen tissue processed for immunofluorescence: A simple procedure to avoid repeat kidney biopsies. Indian J Nephrol. 2013;23:206-10.
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